Product NameLysosomal associated membrane protein 1 (LAMP1), Mouse Monoclonal Antibody
Product DescriptiongoogleMouse anti-Lysosomal associated membrane protein 1 (LAMP1) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Alternative NamesLysosomal Associated Membrane Protein 1, also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA
Application(s)ICC, WB
Antibody HostMouse
Antibody TypeMonoclonal
SpecificityThe antibody reacts with a diffuse band at ~90 kDa to 120 kDa by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry.
Species ReactivityHuman
Immunogen DescriptionRecombinant LAMP1 expressed and purified from E. coli.
Product DescriptionMouse anti-Lysosomal associated membrane protein 1 (LAMP1) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Application(s)ICC, WB
Application DetailsWestern Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:2,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetLysosomal associated membrane protein 1 (LAMP1)
SpecificityThe antibody reacts with a diffuse band at ~90 kDa to 120 kDa by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG1
Clone NameLAMP1
ConjugateUnconjugated
Immunogen DescriptionRecombinant LAMP1 expressed and purified from E. coli.
Purity DescriptionProtein G purified
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesLysosomal Associated Membrane Protein 1, also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA
Scientific BackgroundLAMP1 (Lysosomal Associated Membrane Protein 1, also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA) is a protein primarily associated with the lysosomal membrane. In a typical cell LAMP1 is associated with spherical vesicles located next to the nucleus and the microtubule organizing center (1). LAMP1 is found on the cell surface of lymphocytes undergoing degranulation, a process in which cytoplasmic vesicles fuse with the plasma membrane, and this phenomena resulted in discovery of LAMP1 as a CD protein. The LAMP1 protein has a large N-terminal region which is inside the lysosome, hence topologically external to the cell, which is often referred to as the lumenal domain (2). The lumenal domain consists of two homologous globular segments separated by a proline rich sequence. Next there is a single membrane spanning domain and a short 11 amino acid C-terminal cytoplasmic tail. This tail region contains, at the extreme C-terminus, a so-called YXXI motif which is responsible for the sorting of the intact molecule to the endosome and lysozome, where Y = tyrosine, I = isoleucine and X = almost any amino acid (3). This motif is found in several other lysosomal proteins, where it functions in the same way. There are 417 amino acids in the human LAMP1 molecule, giving a native molecular weight of 44.8 kDa. However the N-terminal lumenal segment of LAMP1 is very heavily and variably glycosylated due to the presence of 18 N-linked glycosylation sites, so that on SDS-PAGE and on Western blots the protein runs as a diffuse band at 90-120 kDa. Antibodies to LAMP1 are therefore excellent markers of lysosomes in mammalian cells, though some LAMP1 may also be seen on late endosomes and on the plasma membrane.
Western blot of HeLA cell crude extract stained with M-1652-100. The antibody binds to a diffuse band running at between 90 and 120 kDa.
HeLa cell staining with M-1652-100 (green), and counterstained with chicken polyclonal antibody to Lamin A/C (green) and DNA (blue). The LAMP1 antibody reveals strong cytoplasmic staining of lysosomes and early endosomes, while the Lamin A/C antibody binds to the nuclear lamina. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.
General ReferencesMatteoni, R. and Kreiss, T. E. Translocation and clustering of endosomes and lysosomes depends on microtubules. J. Cell Biol. 105:1253-1265 (1987). Howe CL, Granger BL, Hull M, Green SA, Gabel CA, Helenius A, Mellman I. Derived protein sequence, oligosaccharides, and membrane insertion of the 120- kDa lysosomal membrane glycoprotein (lgp120): identification of a highly conserved family of lysosomal membrane glycoproteins. Proc Natl Acad Sci U S A. 85:7577-81 (1988). Rohrer J, Schweizer A, Russell D, Kornfeld S. The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. J Cell Biol. 132:565-76 (1996).