Autophagy-related protein 10 (ATG10), Rabbit Polyclonal Antibody

Product Info

Product Name Autophagy-related protein 10 (ATG10), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Autophagy-related protein 10 (ATG10) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Alternative Names Autophagy-related protein 10; APG10-like; ATG10
Application(s) IHC-Frozen
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity IHC, WB and ELISA confirmed the specificity for ATG10. Human. Other species not yet tested.
Species Reactivity Human
Immunogen Description A peptide (CLKNSQKINKNV) corresponding to the C-terminal of human ATG10 protein has been used as the immunogen.
Conjugate Unconjugated
Purity Description Whole serum
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Autophagy-related protein 10 (ATG10) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Application(s) IHC-Frozen
Application Details IHC, immunofluorescence. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Autophagy-related protein 10 (ATG10)
Specificity IHC, WB and ELISA confirmed the specificity for ATG10. Human. Other species not yet tested.
Target Host Species Human
Species Reactivity Human
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description A peptide (CLKNSQKINKNV) corresponding to the C-terminal of human ATG10 protein has been used as the immunogen.
Purity Description Whole serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at minus 20ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Autophagy-related protein 10; APG10-like; ATG10
Uniprot Number Q9H0Y0
Uniprot Number/Name Q9H0Y0 (ATG10_HUMAN)
Scientific Background ATG10 plays a role in autophagy. It is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugations and modificatioon of a soluble form of MAPLC3 1A, a homolog of yeast ATG8, to a membrane bound form. It is also able to directly interact either with ATG5 or ATG7.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Confocal microscopy on immunofluorescently detected ATG10 in cytospin-isolated human white blood cells using Rabbit antibody to ATG10: whole serum (R-145-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 Alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X.

Citations & References

Specific References Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061.
General References Wan D. et al. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729 (2004)
Ota T. et al. Nat. Genet. 36:40-45(2004)
The MGC Project Team. Genome Res. 14:2121-2127(2004).