Product NameAutophagy-related protein 12 (ATG12), Rabbit Polyclonal Antibody
Product DescriptiongoogleRabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative NamesAutophagy-related protein 12; APG12-like; APG 12; ATG12; APG12; APG12L
Application(s)IHC-Frozen, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityIHC and WB confirmed the specificity for ATG12. Human, rat
Species ReactivityHuman, Rat
Immunogen DescriptionA synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Product DescriptionRabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s)IHC-Frozen, WB
Application DetailsIHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAutophagy-related protein 12 (ATG12)
SpecificityIHC and WB confirmed the specificity for ATG12. Human, rat
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Purity DescriptionWhole serum
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAutophagy-related protein 12; APG12-like; APG 12; ATG12; APG12; APG12L
Scientific BackgroundFUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family.
Confocal microscopy on stained ATG12 using Rabbit antibody to ATG12: whole serum (R-112-100) in paraffin embedded human brian section (midfrontal cortex, Alzheimer case).