Autophagy-related protein 16-1 (ATG16 L1), Rabbit Polyclonal Antibody

Product Info

Product Name Autophagy-related protein 16-1 (ATG16 L1), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Autophagy-related protein 16-1 (ATG16 L1) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA, ICC.
Alternative Names Autophagy-related protein 16-1; APG16-like 1; ATG16L1
Application(s) ELISA, ICC, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity Detects human protein via non-reduced WB, and ELISA Human, rat. Other species not yet tested.
Species Reactivity Human, Rat
Immunogen Description N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein has been used as the immunogen.
Conjugate Unconjugated
Purity Description Whole serum
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Autophagy-related protein 16-1 (ATG16 L1) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA, ICC.
Application(s) ELISA, ICC, WB
Application Details Non-reduced western blot: 1:100; IP: 1-50-1:100 (5-10 μL/500uL lysate); ELISA: 1:2000-1:3000 direct detection only, recombinant immunogen.
Target Autophagy-related protein 16-1 (ATG16 L1)
Specificity Detects human protein via non-reduced WB, and ELISA Human, rat. Other species not yet tested.
Target Host Species Human
Species Reactivity Human, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein has been used as the immunogen.
Purity Description Whole serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Autophagy-related protein 16-1; APG16-like 1; ATG16L1
Uniprot Number Q676U5
Uniprot Number/Name Q676U5 (A16L1_HUMAN)
Scientific Background ATG16 L1 plays an essential role in autophagy. It is a homooligomer ant interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). APG16 L1 localises to the cytoplasm, in particularly to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. There are 5 named isoforms produced by alternative splicing. Genetic variation within ATG16 L1 are associated with susceptibility to inflammatory bowel disease type 1.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Atg16 L1 detected in cytospun HL60 cells by fluorescence confocal microscopy using antibody R-158-100. HL60 cells were fixed with acetone 90 seconds; primary antibody was diluted 1:100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1:100 Alexa 546 conjugate) for 30 minutes. Cells stained for Atg16 L1 appear in red. The cells were then counter-stained with Hoechst Dye (blue colour).
Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to ATG16 L1: whole serum (R-158-100).

Western blot (non-reduced) on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to ATG16 L1: whole serum (R-158-100) at a dilution of 1: 100 (ECL).

Citations & References

Specific References Cooney R et al (2009). NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nat. Med. Dec 6 2009
Scharl M. et al (2011). Protein tyrosine phosphatase nonreceptor type 2 regulates autophagosome formation in human intestinal cells. Inflamm Bowel Dis. 2011 Oct 10.
General References Zheng H, et al. DNA Seq. 15:303-305 (2004).
Clark H.F, et al. Genome Res. 13:2265-2270 (2003).