Autophagy-related protein 4 homolog B (APG4B), Rabbit Polyclonal Antibody

Product Info

Product Name Autophagy-related protein 4 homolog B (APG4B), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Autophagy-related protein 4 homolog B (APG4B) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative Names Cysteine protease ATG4B; Autophagy-related protein 4 homolog B; hAPG4B; Autophagin-1; Autophagy-related cysteine endopeptidase 1; AUT-like 1 cysteine endopeptidase; ATG4B; APG4B; AUTL1; KIAA0943;
Application(s) IHC-Frozen, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity IHC, WB and ELISA confirmed the specificity for ATG4B. Human, rat. Other species not yet tested.
Species Reactivity Human, Rat
Immunogen Description A synthetic peptide (CLERFFDSEDED) corresponding to the C-terminus of human ATG4B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from of ATG4B protein in mouse and rat.
Conjugate Unconjugated
Purity Description Whole serum
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Autophagy-related protein 4 homolog B (APG4B) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s) IHC-Frozen, WB
Application Details IHC, immunofluorescence, WB. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Autophagy-related protein 4 homolog B (APG4B)
Specificity IHC, WB and ELISA confirmed the specificity for ATG4B. Human, rat. Other species not yet tested.
Target Host Species Human
Species Reactivity Human, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description A synthetic peptide (CLERFFDSEDED) corresponding to the C-terminus of human ATG4B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from of ATG4B protein in mouse and rat.
Purity Description Whole serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Cysteine protease ATG4B; Autophagy-related protein 4 homolog B; hAPG4B; Autophagin-1; Autophagy-related cysteine endopeptidase 1; AUT-like 1 cysteine endopeptidase; ATG4B; APG4B; AUTL1; KIAA0943;
Uniprot Number Q9Y4P1
Uniprot Number/Name Q9Y4P1 (ATG4B_HUMAN)
Scientific Background FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. SIMILARITY: Belongs to the peptidase C54 family.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to ATG4B (APG4B): whole serum (R-157-100) at a dilution of 1:100 (ECL)
Western blot on 293 cell lysate using Rabbit antibody to ATG4B (APG4B): whole serum (R-157-100) at a dilution of 1:100.

Citations & References

Specific References A. J. Smuder et al (2011) Exercise protects against doxorubicin-induced markers of autophagy signaling in skeletal muscle. J Appl Physiol. 2011 Jul 21
General References Marino G, et al. J. Biol. Chem. 278:3671-3678(2003).
Kabeya Y, et al. J. Cell Sci. 117:2805-2812(2004).
Nagase T, et al. DNA Res. 6:63-70(1999).
Ota T, et al. Nat. Genet. 36:40-45(2004).
Sugawara K, et al. J. Biol. Chem. 280:40058-40065(2005).
Kumanomidou T, et al. J. Mol. Biol. 355:612-618(2006).