SpecificityIHC, ELISA and WB confirmed the specificity for ATG4C. Human, rat. Other species not yet tested.
Species ReactivityHuman, Rat
Immunogen DescriptionA synthetic peptide (CTVYNSDVIDKQ) corresponding to the C-terminal of human ATG4C protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG4C protein in mouse and rat.
Product DescriptionRabbit anti-Autophagy-related protein 4 homolog C (ATG4C) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s)IHC-Frozen, WB
Application DetailsIHC, immunofluorescence and WB. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAutophagy-related protein 4 homolog C (ATG4C)
SpecificityIHC, ELISA and WB confirmed the specificity for ATG4C. Human, rat. Other species not yet tested.
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (CTVYNSDVIDKQ) corresponding to the C-terminal of human ATG4C protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG4C protein in mouse and rat.
Purity DescriptionWhole serum
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundFUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). SIMILARITY: Belongs to the peptidase C54 family.
Confocal microscopy on immunofluorescently detected APG4C in HL60 cell line using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG4C appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100).
Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1: 100 (ECL).
Western blot under reducing conditions on Olfactory cell line (Odora) lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.
Western blot under reducing conditions on rat liver lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.
Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.
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