Autophagy protein 5 (ATG5), Rabbit Polyclonal Antibody

Product Info

Product Name Autophagy protein 5 (ATG5), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative Names Autophagy protein 5; APG5-like; APG 5; Apoptosis-specific protein; APG5; ATG5; APG5L; ASP
Application(s) IHC-Frozen, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity IHC and WB confirmed the specificity for ATG5. Human, not yet tested in other species.
Species Reactivity Human
Immunogen Description A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B has been used to orchestrate/boost the immune response.
Conjugate Unconjugated
Purity Description Whole serum
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s) IHC-Frozen, WB
Application Details IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Autophagy protein 5 (ATG5)
Specificity IHC and WB confirmed the specificity for ATG5. Human, not yet tested in other species.
Target Host Species Human
Species Reactivity Human
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B has been used to orchestrate/boost the immune response.
Purity Description Whole serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Autophagy protein 5; APG5-like; APG 5; Apoptosis-specific protein; APG5; ATG5; APG5L; ASP
Uniprot Number Q9H1Y0
Uniprot Number/Name Q9H1Y0 (ATG5_HUMAN)
Scientific Background FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Confocal microscopy on stained ATG5 using Rabbit antibody to ATG5: whole serum (R-111-100) in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case). ATG5 appears green. Red staining is p25.
DAB staining of ATG5 in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case) using Rabbit antibody to ATG5: whole serum (R-111-100).

Western blot on non-reduced human Neutrophils using Rabbit antibody to ATG5: whole serum (R-111-100) at a dilution of 1:100. The detected band has an apparent molecular weight of 49 kDa corresponding to the ATG5-ATG12 complex molecule.

Citations & References

Specific References E.N. Wilson et al (2011) A Switch Between Cytoprotective and Cytotoxic Autophagy in the Radiosensitization of Breast Tumor Cells by Chloroquine and Vitamin D. Horm Cancer. 2011 Sep 2.

Bristol ML et al (2012) Dual functions of autophagy in the response of breast tumor cells to radiation: cytoprotective autophagy with radiation alone and cytotoxic autophagy in radiosensitization by vitamin D 3. Autophagy. 2012 May 1;8(5):739-53.

Guido C et al (2012) Metabolic reprogramming of cancer-associated fibroblasts by TGF-β drives tumor growth: connecting TGF-_ signaling with "Warburg-like" cancer metabolism and L-lactate production. Cell Cycle. 2012 Aug 15;11(16):3019-35.
General References Mizushima, N et al. (2003) Int J Biochem Cell Biol. 35(5), 553-61
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-(2005)
Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-4(2005)
Greenberg JT. Dev Cell. 8(6):799-80(2005)