Autophagy-related protein 7 (ATG7), Rabbit Polyclonal Antibody

Product Info

Product Name Autophagy-related protein 7 (ATG7), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Autophagy-related protein 7 (ATG7) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Alternative Names Autophagy-related protein 7; Ubiquitin-activating enzyme E1-like protein; hAGP7; APG7-like; ATG7; APG7L
Application(s) IHC-Frozen
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity IF, WB and ELISA confirmed the specificity for ATG7. Human, rat. Other species not yet tested.
Species Reactivity Human, Rat
Immunogen Description A synthetic peptide (DSTRDRTLDQQC) corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Conjugate Unconjugated
Purity Description Whole serum
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Autophagy-related protein 7 (ATG7) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Application(s) IHC-Frozen
Application Details Non-reduced western blot : 1:100; IF: acetone or 3.7% PFA fixed cells (HJ Lim et al 2013); 1:100; Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Autophagy-related protein 7 (ATG7)
Specificity IF, WB and ELISA confirmed the specificity for ATG7. Human, rat. Other species not yet tested.
Target Host Species Human
Species Reactivity Human, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description A synthetic peptide (DSTRDRTLDQQC) corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Purity Description Whole serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Autophagy-related protein 7; Ubiquitin-activating enzyme E1-like protein; hAGP7; APG7-like; ATG7; APG7L
Uniprot Number O95352
Uniprot Number/Name O95352 (ATG7_HUMAN)
Scientific Background FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family. In yeast, ATG7 appears to be required for fusion of peroxisomal and vaculuolar membranes.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Confocal microscopy on cytospin-isolated human blood cells. Neutrophils seem to be stained using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG7 appear in red . The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Observations: No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line.
Western blot on Peripheral Blood Mononuclear Cells, non-reduced, (PMBC) lysate using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1:100 (ECL).

Confocal microscopy on immunofluorescently detected APG7 in cytospin-isolated human white blood cells using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells were double-stained for APG7 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.

Citations & References

Specific References Chiu-Wei Chen, et al (2012) Inhibition of autophagy as a therapeutic strategy of iron-induced brain injury after hemorrhage Autophagy, 8(10):1510.
Chien W.S. et al (2011) Suppression of autophagy in rat liver at late stage of polymicrobial sepsis Shock. 2011 Jan 14
Lee J.E. et al (2011) Autophagy Regulates Embryonic Survival During Delayed Implantation Endocrinology. 2011 Mar 1.
Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061.
Oh HA et al (2013) Uncovering a role for endocannabinoid signaling in autophagy in preimplantation mouse embryos. Mol Hum Reprod. 2013 Feb;19(2):93-101.
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