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Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3 B), Rabbit Polyclonal Antibody

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Catalog Number

R-155

Product Info

Product Name Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3 B), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3 B) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative Names Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated protein 1 light chain 3 beta; MAP1A/MAP1B LC3 B; MAP1A/1B light chain 3 B; MAP1 light chain 3-like protein 2; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; APG8b; MAP-1LC3B; MAP1LC3B; MAP1ALC3;
Application(s) IHC-Frozen, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity IHC, WB and ELISA confirmed the specificity for MAP1LC3 B. Human, rat. Other species not yet tested.
Species Reactivity Human, Rat
Immunogen Description A synthetic peptide (FEQRVEDVRLIC) corresponding to the N-terminal of human MAP1LC3 B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from mouse and rat MAP1LC3 B protein.
Conjugate Unconjugated
Purity Description Whole serum
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3 B) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s) IHC-Frozen, WB
Application Details IHC, immunofluorescence, WB. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3 B)
Specificity IHC, WB and ELISA confirmed the specificity for MAP1LC3 B. Human, rat. Other species not yet tested.
Target Host Species Human
Species Reactivity Human, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description A synthetic peptide (FEQRVEDVRLIC) corresponding to the N-terminal of human MAP1LC3 B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from mouse and rat MAP1LC3 B protein.
Purity Description Whole serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated protein 1 light chain 3 beta; MAP1A/MAP1B LC3 B; MAP1A/1B light chain 3 B; MAP1 light chain 3-like protein 2; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; APG8b; MAP-1LC3B; MAP1LC3B; MAP1ALC3;
Uniprot Number Q9GZQ8
Uniprot Number/Name Q9GZQ8 (MLP3B_HUMAN)
Scientific Background MAP1LC3B is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. MAP1LC3B is probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. Cytoplasmic vesicle; autophagosome; autophagosomal membrane; lipid-anchor. LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Western blot under reducing conditions on Olfactory cell line (Odora) lysate using Rabbit antibody to MAP1LC3 B : whole serum (R-155-100) at a dilution of 1:100.
Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to MAP1LC3 B : whole serum (R-155-100) at a dilution of 1:100.

Citations & References

Specific References Theron AE et al. (2013) Molecular crosstalk between apoptosis and autophagy induced by a novel 2-methoxyestradiol analogue in cervical adenocarcinoma cells. Cancer Cell Int. 2013 Aug 27;13(1):87.

Pilchowski R. et al. (2011) Specific Protein Patterns Characterize Metastatic Potential of Advanced Bladder Cancer. J Urol. 2011 Jun 16.
General References Difilippantonio S, et al. Eur. J. Cancer 39:1936-1947(2003).
Yang Y.-S, et al. Endocr. Relat. Cancer 10:621-627(2003).
He H, et al. J. Biol. Chem. 278:29278-29287(2003).
Ota T, et al. Nat. Genet. 36:40-45(2004).
Tanida I, et al. J. Biol. Chem. 279:36268-36276(2004).
Tanida I, et al. J. Biol. Chem. 279:47704-47710(2004).
Tanida I, et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004).