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Ubiquitin, Rabbit Polyclonal Antibody
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Catalog Number
R-182
- Product Name Ubiquitin, Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Ubiquitin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
- Alternative Names RPS27A; UBA52; UBB; UBC
- Application(s) ICC, IHC-Frozen, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been determined by indirect ELISA against ubiquitin conjugated to keyhole limpet haemocyanin. The antibody does not react with kehole limpet haemocyanin alone. Specificity has also been demonstrated by WB against endometrial tissue homogenates. Cross reacts with human, baboon and rat ubiquitin. Other species have not been tested. Ubiquitin protein sequence is 100% conserved in all higher mammals and most eukarotes.
- Species Reactivity Bovine, Human, Other Mammals (Predicted), Primate, Rat
- Immunogen Description Ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins with glutaraldehyde.
- Conjugate Unconjugated
- Purity Description Protein G purified immunoglobulin.
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Ubiquitin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
- Application(s) ICC, IHC-Frozen, WB
- Application Details This antibody is recommended for IHC, WB and immunoprecipitation. This antibody can be used for labelling formalin-fixed, paraffin-embedded tissue sections at a dilution range of 1:150 to 1:300. This antibody is also useful for investigating neurodegenerative diseases such as Alzheimer 's disease and Parkinson's disease. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Ubiquitin
- Specificity The specificity of this antibody has been determined by indirect ELISA against ubiquitin conjugated to keyhole limpet haemocyanin. The antibody does not react with kehole limpet haemocyanin alone. Specificity has also been demonstrated by WB against endometrial tissue homogenates. Cross reacts with human, baboon and rat ubiquitin. Other species have not been tested. Ubiquitin protein sequence is 100% conserved in all higher mammals and most eukarotes.
- Target Host Species Bovine
- Species Reactivity Bovine, Human, Other Mammals (Predicted), Primate, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description Ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins with glutaraldehyde.
- Purity Description Protein G purified immunoglobulin.
- Format Lyophilized from PBS, pH 7.2, 100mM NaCl, 15mM sodium azide.
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 250 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names RPS27A; UBA52; UBB; UBC
- Uniprot Number P62990
- Uniprot Number/Name P62990 (UBIQ_BOVIN)
- Scientific Background Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.