SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide (HRKHLQEIPDLSSNVATSF) corresponding to a region (2-20) from the N-terminus of human S-phase kinase-associated protein 2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human SKP2 (Isoform SKP2_alpha) has a predicted length of 424 residues and MW of 48 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target S-phase kinase-associated protein 2
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (HRKHLQEIPDLSSNVATSF) corresponding to a region (2-20) from the N-terminus of human S-phase kinase-associated protein 2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesF-box protein Skp2; Cyclin A/CDK2-associated protein p45; p45skp2; F-box/LRR-repeat protein 1; SKP2; FBXL1;
Scientific BackgroundS-phase kinase-associated protein 2 (SKP2) is a subunit in the SKP1-CUL1-F-box protein E3 ubiquitin protein ligase complex. This complex mediates the ubiquitination and the subsequent degradation of proteins involved in cell cycle progression, signal transduction and transcription.
Immunohistochemical staining of paraffin-embedded human mammary cancer sections using rabbit polyclonal antibody to human S-phase kinase-associated protein 2 (1-2 µg/ml). Secondary biotinylated goat anti-rabbit IgG antibody (10 µg/ml).