PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK), Rabbit Polyclonal Antibody

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Catalog Number
R-1333

    Product Info

  • Product Name PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; Eif2ak3; Pek; Perk;
  • Application(s) ICC, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity This antiserum is known to recognise both phosphorylated and non phosphorylated mouse PERK.
  • Species Reactivity Mouse
  • Immunogen Description A recombinant peptide from mouse PERK.
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western Blotting (WB) and Immunoprecipitation (IP). A dilution of 1:500 is recommended for WB. A dilution of 30 µL of antibody in a total reaction mixture of 500 µL is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK)
  • Specificity This antiserum is known to recognise both phosphorylated and non phosphorylated mouse PERK.
  • Target Host Species Mouse
  • Species Reactivity Mouse
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description A recombinant peptide from mouse PERK.
  • Purity Description Whole serum
  • Format Liquid. 50% glycerol
  • Storage Instructions Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; Eif2ak3; Pek; Perk;
  • Uniprot Number Q9Z2B5
  • Uniprot Number/Name Q9Z2B5 (E2AK3_MOUSE)
  • Scientific Background PERK (PKR-like ER kinase) is a single-pass type I ER membrane protein with a stress-sensing luminal domain connected by a transmembrane segment to a cytoplasmic-kinase domain.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot using mouse cultured astrocyte extracts at rest (Cntrl) and after 60 mins of ER stress induction by oxygen glucose deprivation (OGD). The left panel membrane was probed with Rabbit polyclonal antibody to mouse PERK: Whole serum (R-1333-100). The right membrane was similarly probed in combination with the antigenic peptide used to generate the antibody. Image taken from Bollo et al (2010) Figure S4.

    Citations & References

  • Specific References Feliziani C. et al (2022) Ca2+ signalling system initiated by Endoplasmic reticulum stress stimulates PERK activation Cell Calcium. 2022 [Epub ahead of print]

    Bollo M. et al (2010) Calcineurin interacts with PERK and dephosphorylates calnexin to relieve ER stress in mammals and frogs PLoS One. 2010 Aug 5;5(8)