Ki-67, Chicken Polyclonal Antibody

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Catalog Number
C-1807

    Product Info

  • Product Name Ki-67, Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Ki-67 Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67
  • Application(s) ICC, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Human, reacts with human only. Does not react with mouse or rat.
  • Species Reactivity Human
  • Immunogen Description Recombinant human Ki-67 protein (mixture of amino acids 1-300 and 1,111-1,490) expressed in and purified from E. coli.
  • Conjugate Unconjugated
  • Purity Description IgY fraction
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Ki-67 Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western blotting (1:2,000-1:5,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Ki-67
  • Specificity Human, reacts with human only. Does not react with mouse or rat.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Recombinant human Ki-67 protein (mixture of amino acids 1-300 and 1,111-1,490) expressed in and purified from E. coli.
  • Purity Description IgY fraction
  • Format Lyophilized from a solution containing PBS buffer pH 7.2-7.6, without preservatives.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67
  • Uniprot Number P46013
  • Uniprot Number/Name P46013 (KI67_HUMAN)
  • Scientific Background Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref: uniprot.org
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: Immunofluorescence analysis of HeLa cells costained with chicken antibody to Ki-67 (1:2,000, red) and a mouse antibody to beta-tubulin. Ki-67 is predominantly localized in nucleoli of cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosomes. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates using chicken antibody to Ki-67 (1:5,000). Double bands above 250 kDa correspond to two major Ki-67 isoforms of molecular weight ~345 and 395 kDa. Smaller fragments of these isoforms are also detected on the blot.

    Citations & References