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Cadherin-2, pan (CDH), Mouse Monoclonal Antibody
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Catalog Number
M-1740
- Product Name Cadherin-2, pan (CDH), Mouse Monoclonal Antibody
- Product Description Mouse anti-Cadherin-2 (CDH) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names CDH; N-cadherin; Neural Cadherin; Cadherin-2; P-cadherin; R-cadherin; B-cadherin; E-cadherin;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit; chicken; snake;
- Species Reactivity Chicken, Human, Mouse, Rabbit, Rat, Snake
- Immunogen Description A synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.
- Conjugate Unconjugated
- Purity Description IgG
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Cadherin-2 (CDH) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human N-Cadherin has a predicted length of 906 residues and MW of 100 kDa. A concentration of 2.0-4.0 µg/mL is recommended for IHC to detect the protein in paraffin embedded tissues. Antigen retrieval by heat is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Cadherin-2 (CDH)
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit; chicken; snake;
- Target Host Species Chicken
- Species Reactivity Chicken, Human, Mouse, Rabbit, Rat, Snake
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG
- Clone Name CH-19
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.
- Purity Description IgG
- Format Lyophilized. Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative.
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At -20°C for one year. After reconstitution, at 2-8°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names CDH; N-cadherin; Neural Cadherin; Cadherin-2; P-cadherin; R-cadherin; B-cadherin; E-cadherin;
- Uniprot Number P10288
- Uniprot Number/Name P10288 (CADH2_CHICK)
- Scientific Background Cadherins are transmembrane glycoproteins that mediate calcium ion-dependent cell adhesion. The cadherin family includes N-, P-, R-, B- and E-cadherins which are found in adherens junctions.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
- Specific References Kawai T and Shimada M (2020). Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. Sci Rep. 10(1):6841; Application: IHC/IF, WB Species: Mouse