Application DetailsImmunohistochemistry (IHC). A concentration of 0.5-1.0 µg/mL is recommended to detect Collagen IV in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCollagen alpha-1 (IV) chain
SpecificityThe specificity of this antibody has been confirmed by IHC against the antigen. Human
Scientific BackgroundCollagen IV is a major constituent of the glomerular basement membranes. It is a heterotrimeric protein composed of 3 alpha subunits encoded by 6 different genes; alpha 1(IV) through alpha 6(IV).