Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human CDC2 (isoform 1) has a predicted length of 297 residues and MW of 34 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues.
TargetCyclin-dependent kinase 1
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; chicken;
Target Host SpeciesXenopus
Species ReactivityChicken, Human, Mouse, Xenopus
Immunogen DescriptionA C-terminal fragment of Xenopus Cyclin-dependent kinase 1 expressed in E. coli
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesCell division control protein 2 homolog; p34 protein kinase; p34cdc2; CDK1; CDC2;
Scientific BackgroundCyclin-dependent kinase 1, also known as cell division control protein 2 homolog (CDC2), belongs to the Ser/Thr protein kinase family. CDC2 is required for entry into S phase of the cell cycle and mitosis. Multiple alternatively spliced variants have been reported for this gene.