Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/mL is recommended for WB. Human Desmin has a predicted length of 470 residues and MW of 54 kDa. A concentration of 2.0-4.0 µg/mL is recommended to detect Desmin in formalin fixed and paraffin embedded tissues. Heat retrieval is necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundDesmin are class-III intermediate filaments expressed in smooth, cardiac and heart muscles. Desmin homopolymers form a stable fibrous network connecting myofibrils to each other and to the plasma membrane.