Blots of mice peripheral nerves blotted with M-1376-250 for use as a western blotting control. Note the single sharp band corresponding to GAPDH.
Left: Staining of GAPDH in HeLa cells with mouse anti-GAPDH antibody (1:100, green) by Immunocytochemistry. Blue: DAPI nuclear stain. The GAPDH antibody produces strong cytoplasmic staining of healthy cells. Right: Western blot analysis of cell lysates probed with mouse antibody to GAPDH (1:2,000). [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6. The GAPDH antibody reveals a single band at about 37 kDa in all cell lines. GAPDH is a "house keeping” protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.