Blots of mice peripheral nerves blotted with M-1376-250 for use as a western blotting control. Note the single sharp band corresponding to GAPDH.
Left: Staining of GAPDH in HeLa cells with mouse anti-GAPDH antibody (1:100, green) by Immunocytochemistry. Blue: DAPI nuclear stain. The GAPDH antibody produces strong cytoplasmic staining of healthy cells. Right: Western blot analysis of cell lysates probed with mouse antibody to GAPDH (1:2,000).  protein standard,  HEK293,  HeLa,  SH-SY5Y,  COS1,  NIH-3T3, and  C6. The GAPDH antibody reveals a single band at about 37 kDa in all cell lines. GAPDH is a "house keeping‚Äù protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.