Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), Mouse Monoclonal Antibody

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Catalog Number
M-1376

    Product Info

  • Product Name Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
  • Alternative Names Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; G3PDH; GPDH;
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Bovine, Porcine, Chicken
  • Species Reactivity Bovine, Chicken, Human, Mouse, Pig, Rat
  • Immunogen Description Purified pig GAPDH
  • Conjugate Unconjugated
  • Purity Description Purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 is recommended for WB. Human GAPDH has a predicted length of 335 residues and a MW of 36 kDa. A dilution of 1:100 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)
  • Specificity The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Bovine, Porcine, Chicken
  • Target Host Species Pig
  • Species Reactivity Bovine, Chicken, Human, Mouse, Pig, Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgM
  • Clone Name 1D4
  • Conjugate Unconjugated
  • Immunogen Description Purified pig GAPDH
  • Purity Description Purified
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 250 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; G3PDH; GPDH;
  • Uniprot Number P00355
  • Uniprot Number/Name P00355 (G3P_PIG)
  • Scientific Background Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Human neuroblastoma SH-SY5Y cells stained with Mouse monoclonal antibody to Glyceraldehyde 3-Phosphate Dehydrogenase [1D4] M-1376-250 (green), chicken antibody to neurofilament H C-1386-50 (red) and DNA (blue). The antibody reveals strong cytoplasmic staining for GAPDH.

  • Blots of mice peripheral nerves blotted with M-1376-250 for use as a western blotting control. Note the single sharp band corresponding to GAPDH.


    Left: Staining of GAPDH in HeLa cells with mouse anti-GAPDH antibody (1:100, green) by Immunocytochemistry. Blue: DAPI nuclear stain. The GAPDH antibody produces strong cytoplasmic staining of healthy cells. Right: Western blot analysis of cell lysates probed with mouse antibody to GAPDH (1:2,000). [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6. The GAPDH antibody reveals a single band at about 37 kDa in all cell lines. GAPDH is a "house keeping” protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.