Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human P Glycoprotein has a predicted length of 1280 residues and MW of 141 kDa. A concentration of 1.0-2.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 1.0-2.0 µg/mL is recommended for IHC to detect the protein in formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetP-glycoprotein 1
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NamePG-13
ConjugateUnconjugated
Immunogen DescriptionHuman and hamster drug-resistant whole cells and crude plasma membranes
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesMultidrug resistance protein 1; ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD243; ABCB1; MDR1; PGY1;
Scientific BackgroundP Glycoprotein belongs to the MDR/TAP subfamily of ATP-binding cassette (ABC) transporters. P Glycoprotein is an ATP-dependent drug efflux pump expressed in liver, kidney, small intestine and brain. It is responsible for decreased drug accumulation in multidrug-resistant cells. Genetic variation in P Glycoprotein is thought to play a role in patients who do not respond to drug treatment. P Glycoprotein also functions as a transporter in the blood-brain barrier.