Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-2.0 µg/mL is recommended for WB. Chicken HSP25 has a predicted length of 172 residues and MW of 19 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 1.0-2.0 µg/mL is recommended to detect HSP25 in formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetHeat shock protein 25 (HSP-25)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Target Host SpeciesTurkey
Species ReactivityHuman, Turkey
Immunogen DescriptionPartially purified inhibitor of actin polymerization (IAP) protein from Turkey Gizzard smooth muscle
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesHeat shock 25 kDa protein 1; HSP25;