Vimentin, Mouse Monoclonal Antibody

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Catalog Number
M-1161

    Product Info

  • Product Name Vimentin, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Vimentin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names VIM;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
  • Species Reactivity Human, Mouse, Pig, Rat
  • Immunogen Description Pig eye lens vimentin
  • Conjugate Unconjugated
  • Purity Description IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Vimentin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human vimentin (precursor) has a predicted length of 466 residues and MW of 54 kDa. A concentration of 1.5-2.5 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Vimentin
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
  • Target Host Species Pig
  • Species Reactivity Human, Mouse, Pig, Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name VMT-24
  • Conjugate Unconjugated
  • Immunogen Description Pig eye lens vimentin
  • Purity Description IgG
  • Format Lyophilized from 1.2% sodium acetate, 2 mg BSA, 0.01 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names VIM;
  • Uniprot Number P02543
  • Uniprot Number/Name P02543 (VIME_PIG)
  • Scientific Background Vimentins are class-III intermediate filaments specific to mesenchymal tissue. Vimentin is an important cytoskeletal component responsible for maintaining cell integrity and has a probable role in the intracellular transport of proteins such as lipoproteins between the nucleus and plasma membrane. Immunohistochemical staining for Vimentin is characteristic of sarcomas.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

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