Nestin, Chicken Polyclonal Antibody

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Catalog Number
C-1812

    Product Info

  • Product Name Nestin, Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Nestin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names NES;
  • Application(s) ICC, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Human,reacts with human and rat. Other species not tested.
  • Species Reactivity Human, Rat
  • Immunogen Description Part of recombinant human protein (amino acids 315-630).
  • Conjugate Unconjugated
  • Purity Description IgY fraction
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Nestin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Nestin
  • Specificity Human,reacts with human and rat. Other species not tested.
  • Target Host Species Human
  • Species Reactivity Human, Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Part of recombinant human protein (amino acids 315-630).
  • Purity Description IgY fraction
  • Format Lyophilized from a solution containing PBS buffer pH 7.2-7.6, without preservatives.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names NES;
  • Uniprot Number P48681
  • Uniprot Number/Name P48681 (NEST_HUMAN)
  • Scientific Background Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Ref: uniprot.org
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: SH-SY5Y neuroblastoma cells stained with chicken antibody to Nestin (green, 1:2,000) and rabbit antibody to Vimentin (R-1699-100, red, 1:2,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in rat C6 (Lane 1) and human SH-SY5Y (Lane 2) cell lysates. The antibody (1:1,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.

  • Nestin staining (red) in human mesenchymal stem cells by Immunocytochemistry. Primary antibody dilution: 1:3,000. Image courtesy of QBM Cell Science.


    Nestin staining (red) in normal human atrial cardiac fibroblasts (3DIV) by Immunocytochemistry, using chicken antibody to nestin, C-1812-50. Green: beta-tubulin (left), anti-fibroblast antibody (right). Blue: cell nuclei. Image courtesy of QBM Cell Science.

    Citations & References