Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1), Chicken Polyclonal Antibody

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Catalog Number
C-1398

    Product Info

  • Product Name Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
  • Alternative Names Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin-1; Rotamase Pin1; PIN1;
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~21 kDa Pin1 protein. Human, Rat, Mouse and Feline. Predicted to react with other mammalian tissue.
  • Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
  • Immunogen Description Recombinant full length Peptidylprolyl isomerase (Pin1) purified from E.coli
  • Conjugate Unconjugated
  • Purity Description IgY
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1)
  • Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~21 kDa Pin1 protein. Human, Rat, Mouse and Feline. Predicted to react with other mammalian tissue.
  • Target Host Species Human
  • Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Recombinant full length Peptidylprolyl isomerase (Pin1) purified from E.coli
  • Purity Description IgY
  • Format Lyophilized with 5% trehalose
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin-1; Rotamase Pin1; PIN1;
  • Uniprot Number Q13526
  • Uniprot Number/Name Q13526 (PIN1_HUMAN)
  • Scientific Background The enzyme Peptidylprolyl isomerase (Pin1) is responsible for flipping the proline ring from the cis to trans conformation. This enzyme regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity (ref: SWISSPROT). Pin1 is concentrated in the nucleus in small punctate structures and is particularly obvious in tumor cells.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • HeLa cells stained with Chicken polyclonal antibody to Peptidylprolyl isomerase (1:1,000 dilution, green) and Mouse monoclonal antibody to Fibrillarin (Nop1p) M-1372-250 (red). Peptidylprolyl isomerase (Pin-1) stains the nuclear matrix and, much more faintly, the cytoplasm. The fibrillarin antibody marks nucleoli.

    Citations & References