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Rho-associated protein kinase 2 (ROCK2), Rabbit Polyclonal Antibody
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Catalog Number
R-1789
- Product Name Rho-associated protein kinase 2 (ROCK2), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Rho-associated protein kinase 2 (ROCK2) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, WB.
- Alternative Names Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity Human, reacts with human, rat, mouse..
- Species Reactivity Human, Mouse, Rat
- Immunogen Description E.coli-derived human ROCK2 recombinant protein (amino acids E652-D923).
- Conjugate Unconjugated
- Purity Description Affinity purified
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Rho-associated protein kinase 2 (ROCK2) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, WB.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Rho-associated protein kinase 2 has a predicted length of 1388 amino acids and MW of 161 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Rho-associated protein kinase 2 (ROCK2)
- Specificity Human, reacts with human, rat, mouse..
- Target Host Species Human
- Species Reactivity Human, Mouse, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description E.coli-derived human ROCK2 recombinant protein (amino acids E652-D923).
- Purity Description Affinity purified
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2
- Uniprot Number P20656
- Uniprot Number/Name P20656 (RIP6_SAPOF)
- Scientific Background Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/mLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca2+ sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
