SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Species ReactivityHuman
Immunogen DescriptionA synthetic peptide (RGVPQIEVTFDIDANGILN) corresponding to a region (469-487) from human Heat Shock 70 kDa Protein 1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human Heat Shock 70 kDa Protein 1 has a predicted length of 641 residues and MW of 70 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetHeat Shock 70 kDa Protein 1 (HSP70-1)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (RGVPQIEVTFDIDANGILN) corresponding to a region (469-487) from human Heat Shock 70 kDa Protein 1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesHeat shock 70 kDa protein 1; HSP70.1; HSP70-1; HSP70-2; HSPA1A; HSPA1; HSPA1B; FLJ54328;
Scientific BackgroundHeat Shock 70 kDa protein 1 belongs to the Heat Shock protein 70 family. This chaperone protein is expressed in response to heat shock and stabilizes existing proteins against aggregation as well as mediating the folding of newly translated proteins.