Application DetailsWestern Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human Interleukin 10 (precursor) has a predicted length of 178 amino acids and MW of 21 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetInterleukin 10 (IL-10)
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human;
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to a region (46-60 aa) from human Interleukin 10.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesInterleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10;
Scientific BackgroundInterleukin 10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells (Ref: SWISSPROT).