SpecificityThe specificity of this antibody has been confirmed by WB (Human, Rat) and IHC (Human) against the antigen. Human; rat; predicted to react with mouse due to sequence homology
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to a region (689-705 aa) from human Matrix metallopeptidase 9.
Application DetailsWestern Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/mL is recommended for WB. Human MMP-9 (precursor) has a predicted length of 707 amino acids and MW of 78 kDa. A concentration of 1.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMatrix metallopeptidase 9 (MMP-9)
SpecificityThe specificity of this antibody has been confirmed by WB (Human, Rat) and IHC (Human) against the antigen. Human; rat; predicted to react with mouse due to sequence homology
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to a region (689-705 aa) from human Matrix metallopeptidase 9.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesMatrix metallopeptidase 9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; MMP9; CLG4B;
Scientific BackgroundMatrix metallopeptidase 9 (MMP-9) cleaves collagen types IV and type V and gelatin types 1 and V. MMP-9 has a possible role in local proteolysis of the extracellular matrix and in leukocyte migration (Ref: SWISSPROT).