Matrix metalloproteinase (MMP-2), Rabbit Polyclonal Antibody

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Catalog Number
R-1735
Discontinued

    Product Info

  • Product Name Matrix metalloproteinase (MMP-2), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Matrix metalloproteinase (MMP-2) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names 72 kDa type IV collagenase; EC 3.4.24.24; 72 kDa gelatinase; Matrix metalloproteinase-2; MMP-2; Gelatinase A; TBE-1; MMP2; CLG4A;
  • Application(s) ELISA, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity paraffin sections), rat (WB, IHC paraffin and frozen sections) and mouse (IHC paraffin and frozen sections).
  • Species Reactivity Human, Mouse (Predicted), Rat (Predicted)
  • Immunogen Description A synthetic peptide corresponding to a region (138-153) at the N-terminus from human Matrix metalloproteinase-2.
  • Conjugate Unconjugated
  • Purity Description Affinity purified on immunogen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Matrix metalloproteinase (MMP-2) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details Western Blotting (WB) and ELISA. A concentration of 0.1-0.5 µg/mL is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Matrix metalloproteinase (MMP-2)
  • Specificity paraffin sections), rat (WB, IHC paraffin and frozen sections) and mouse (IHC paraffin and frozen sections).
  • Target Host Species Human
  • Species Reactivity Human, Mouse (Predicted), Rat (Predicted)
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide corresponding to a region (138-153) at the N-terminus from human Matrix metalloproteinase-2.
  • Purity Description Affinity purified on immunogen column
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution, store at 2-8°C short-term (1 month). Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names 72 kDa type IV collagenase; EC 3.4.24.24; 72 kDa gelatinase; Matrix metalloproteinase-2; MMP-2; Gelatinase A; TBE-1; MMP2; CLG4A;
  • Uniprot Number P08253
  • Uniprot Number/Name P08253 (MMP2_HUMAN)
  • Scientific Background The matrix metalloproteinases (MMPs) are a large family of zinc endopeptidases. All MMPs are synthesized as inactive proenzymes. The activation of these proenzymes is a critical step that leads to degradation of extracellular matrix components such as fibronectin and collagen type III. MMP2 is activated by other MMPs such as MMP14 and MMP16. MMP2 is known to degrade collagen types IV, V, VII and X as well as gelatin type I.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot analysis of MMP2 protein loaded at 1 ng. The antibody was used at 0.5 µg/mL with overnight incubation at 4°C. A specific band is detected for MMP2 at the expected molecular weight of ~72 kD.

    Citations & References