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DNA topoisomerase 2-alpha, Rabbit Polyclonal Antibody
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Catalog Number
R-1109
- Product Name DNA topoisomerase 2-alpha, Rabbit Polyclonal Antibody
- Product Description Rabbit anti-DNA topoisomerase 2-alpha Polyclonal Antibody (Unconjugated), suitable for WB.
- Alternative Names EC 5.99.1.3; DNA topoisomerase II; alpha isozyme; TOP2A; TOP2;
- Application(s) WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human; rat;
- Species Reactivity Human, Rat
- Immunogen Description A synthetic peptide (RKPSTSDDSDSNFEK) corresponding to a region (1466-1480) from human DNA topoisomerase 2-alpha. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-DNA topoisomerase 2-alpha Polyclonal Antibody (Unconjugated), suitable for WB.
- Application(s) WB
- Application Details Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human TOP2A (isoform 1) has a predicted length of 1,531 residues and MW of 174 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target DNA topoisomerase 2-alpha
- Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human; rat;
- Target Host Species Human
- Species Reactivity Human, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (RKPSTSDDSDSNFEK) corresponding to a region (1466-1480) from human DNA topoisomerase 2-alpha. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names EC 5.99.1.3; DNA topoisomerase II; alpha isozyme; TOP2A; TOP2;
- Uniprot Number P11388
- Uniprot Number/Name P11388 (TOP2A_HUMAN)
- Scientific Background DNA topoisomerase 2 catalyzes the transient breaking and rejoining of double stranded DNA during events such as DNA synthesis and transcription. Two forms of this enzyme exist, alpha and beta. DNA topoisomerase 2 alpha (TOP2A) is localized to chromosome 17 and the beta gene is localized to chromosome 3. At least 4 isoforms of TOP2A are produced by alternate splicing.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.