SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human (WB); rat (WB, IHC-P); predicted to react with mouse due to sequence homology;
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (YRPVIDKTLNEADCAT) corresponding to a region (641-656 aa) from the C-terminus of human Succinate dehydrogenase [ubiquinone] flavoprotein subunit (SDNA).
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human SDHA (precursor) has a predicted length of 664 residues and MW of 73 kDa. A concentration of 2.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human (WB); rat (WB, IHC-P); predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (YRPVIDKTLNEADCAT) corresponding to a region (641-656 aa) from the C-terminus of human Succinate dehydrogenase [ubiquinone] flavoprotein subunit (SDNA).
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSuccinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp; SDH2; SDHF; DHSA;
Scientific BackgroundFlavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (Ref: SWISSPROT).