Succinate dehydrogenase [ubiquinone] flavoprotein subunit, Rabbit Polyclonal Antibody

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Catalog Number
R-1348
Discontinued

    Product Info

  • Product Name Succinate dehydrogenase [ubiquinone] flavoprotein subunit, Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Succinate dehydrogenase [ubiquinone] flavoprotein subunit Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp; SDH2; SDHF; DHSA;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human (WB); rat (WB, IHC-P); predicted to react with mouse due to sequence homology;
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Immunogen Description A synthetic peptide (YRPVIDKTLNEADCAT) corresponding to a region (641-656 aa) from the C-terminus of human Succinate dehydrogenase [ubiquinone] flavoprotein subunit (SDNA).
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Succinate dehydrogenase [ubiquinone] flavoprotein subunit Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human SDHA (precursor) has a predicted length of 664 residues and MW of 73 kDa. A concentration of 2.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Succinate dehydrogenase [ubiquinone] flavoprotein subunit
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human (WB); rat (WB, IHC-P); predicted to react with mouse due to sequence homology;
  • Target Host Species Human
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (YRPVIDKTLNEADCAT) corresponding to a region (641-656 aa) from the C-terminus of human Succinate dehydrogenase [ubiquinone] flavoprotein subunit (SDNA).
  • Sequence YRPVIDKTLNEADCAT
  • Purity Description Affinity purified
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp; SDH2; SDHF; DHSA;
  • Uniprot Number P31040
  • Uniprot Number/Name P31040 (SDHA_HUMAN)
  • Scientific Background Flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (Ref: SWISSPROT).
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot using Rabbit polyclonal antibody to SDHA:
    Lane 1: MCF-7 Whole cell lysate
    Lane 2: Hela Whole cell lysate
    Lane 3: Jurkat Whole cell lysate
    Lane 4: HT1080 Whole cell lysate
    Lane 5: Colo320 Whole cell lysate

    Citations & References