Application DetailsWestern Blotting (WB). A concentration of 2.0-4.0 µg/mL is recommended for WB. Human p27 KIP 1 has a predicted length of 198 residues and MW of 22 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCyclin-dependent kinase inhibitor 1B
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
Target Host SpeciesRat
Species ReactivityHuman, Mouse, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameIMD-27
ConjugateUnconjugated
Immunogen DescriptionRecombinant full length rodent p27 KIP 1 protein
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCyclin-dependent kinase inhibitor p27 (p27 KIP 1) is cell cycle regulatory mitotic inhibitor of cdk activity and an important regulator of cell cycle progression.