Application DetailsWestern Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human CDK4 has a predicted length of 303 residues and MW of 34 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCyclin-dependent kinase 4 (CDK4)
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameIML-4
ConjugateUnconjugated
Immunogen DescriptionRecombinant human Cyclin-dependent kinase 4 (CDK4)
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesCell division protein kinase 4; PSK-J3; CDK4;
Scientific BackgroundCyclin-dependent kinase 4 (CDK4) belongs to the Ser/Thr protein kinase family. CDK4 is a subunit of the protein kinase complex that is important for cell cycle G1 phase progression. Mutations in CDK4 have been implicated in tumor formation. Multiple alternatively spliced variants and polyadenylation sites have been reported for this gene.