Galectin-3 (Gal-3), Mouse Monoclonal Antibody

As low as US$317.00
Only %1 left
Catalog Number
M-1574

    Product Info

  • Product Name Galectin-3 (Gal-3), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Galectin-3 (Gal-3) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names Macrophage galactose-specific lectin; MAC2; galactoside-binding protein; GALBP; Galectin 3; Gal 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3;
  • Application(s) ICC, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Human; Mouse; Rat; predicted to react with other species due to sequence homology;
  • Species Reactivity Human, Mouse, Other Mammals (Predicted), Rat
  • Immunogen Description Full length recombinant Galectin-3 expressed in and purified from E. coli.
  • Conjugate Unconjugated
  • Purity Description Protein G purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Galectin-3 (Gal-3) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details WB, ICC. Suggested dilution of at least 1:1,000 for ICC. Suggested dilution of 1:2,000 or lower for WB. Optimal concentrations/dilutions should be determined by the end-user.
  • Target Galectin-3 (Gal-3)
  • Specificity Human; Mouse; Rat; predicted to react with other species due to sequence homology;
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Other Mammals (Predicted), Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG1
  • Clone Name 5C21
  • Conjugate Unconjugated
  • Immunogen Description Full length recombinant Galectin-3 expressed in and purified from E. coli.
  • Purity Description Protein G purified
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Macrophage galactose-specific lectin; MAC2; galactoside-binding protein; GALBP; Galectin 3; Gal 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3;
  • Uniprot Number P17931
  • Uniprot Number/Name P17931 (LEG3_HUMAN)
  • Scientific Background Galectin 3 is a lectin with carbohydrate recognition domains (CRD) which bind -galactoside. It is a multifunctional protein expressed both on the cell surface, cytoplasm and nucleus and appears to have roles in specific carbohydrate binding and in the regulation of mRNA splicing.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Image shows rat brain neural cultures stained with Mouse Monoclonal Antibody to Galectin-3 M-1574-100 (green) and DNA (blue). Staining can be seen in several types of glia and lymphocytic cells, including these cells which have the morphology of microglia. Surrounding cells reveal no Galectin-3 staining.

  • Left: Analysis of galectin-3 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Cells were stained with mouse antibody to galectin-3 (red, 1:1,000), and co-stained with chicken antibody to MAP2 (C-1382-50, green, 1:10,000). Blue: Hoechst nuclear stain. The galectin-3 antibody produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while MAP2-antibody labels dendrites and perikarya of mature neurons. Right: Western blot analysis of tissue and cell lysates using mouse antibody to galectin-3 (green, 1:2,000): [1] protein standard, [2] mouse brain lysate; [3] - [7] rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at 30 kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with an antibody to HSP60 (red, lanes 2-8).

    Citations & References