Cellular tumor antigen p53, Mouse Monoclonal Antibody

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Catalog Number
M-1208

    Product Info

  • Product Name Cellular tumor antigen p53, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Cellular tumor antigen p53 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names Cellular tumor antigen p53; Tumor suppressor p53; Phosphoprotein p53; NY-CO-13; TP53; P53;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
  • Species Reactivity Human
  • Immunogen Description Recombinant human wild-type p53 protein
  • Conjugate Unconjugated
  • Purity Description IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Cellular tumor antigen p53 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human p53 (isoform 1) has a predicted length of 393 residues and MW of 44 kDa. A concentration of 0.5-2.0 µg/mL is recommended to detect p53 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Cellular tumor antigen p53
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name IMD-53
  • Conjugate Unconjugated
  • Immunogen Description Recombinant human wild-type p53 protein
  • Purity Description IgG
  • Format Lyophilized from 1.2% sodium acetate, 2 mg BSA, 0.01 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Cellular tumor antigen p53; Tumor suppressor p53; Phosphoprotein p53; NY-CO-13; TP53; P53;
  • Uniprot Number P04637
  • Uniprot Number/Name P04637 (P53_HUMAN)
  • Scientific Background p53 is a DNA-binding protein containing transcription activation, DNA-binding and oligomerization domains. p53 is ubiquitously expressed and responds to a variety of cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair or changes in metabolism. Multiple p53 variants are produced from alternative promoters and alternative splicing.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot using mouse monoclonal antibody to human p53 at a concentration of 1 µg/mL in HL-60 cell lysate.

    Citations & References