Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253), Rabbit Polyclonal Antibody

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Catalog Number
R-1706

    Product Info

  • Product Name Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Application(s) WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Rat Predicted from gene analysis to react with human and mouse alpha-CaMKII.
  • Species Reactivity Rat
  • Immunogen Description A synthetic peptide (NKmLpTINPSC) corresponding to the sequence around Thr253 (AA 249-257) in alpha-CaMKII was synthesized, purified to 95% purity by HPLC, analyzed by mass spectroscopy and coupled to diphtheria toxoid.
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Application(s) WB
  • Application Details Western Blotting (1:200 - 1:1000). Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253)
  • Specificity Rat Predicted from gene analysis to react with human and mouse alpha-CaMKII.
  • Target Host Species Rat
  • Species Reactivity Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (NKmLpTINPSC) corresponding to the sequence around Thr253 (AA 249-257) in alpha-CaMKII was synthesized, purified to 95% purity by HPLC, analyzed by mass spectroscopy and coupled to diphtheria toxoid.
  • Purity Description Whole serum
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Uniprot Number P11275
  • Uniprot Number/Name P11275 (KCC2A_RAT)
  • Scientific Background Calcium/calmodulin-stimulated protein kinase II (CaMKII) is composed of four different chains (alpha, beta, gamma, and delta) and is abundantly expressed in neurons. CaMKII is involved in regulating many aspects of neuronal function, including neurotransmitter synthesis and release, modulation of ion channel activity and cellular transport. The enzymatic function of CaMKII is regulated by its multiple phosphorylation sites and targeting to sub-cellular locations through interactions with protein binding partners. Phosphorylation of Thr253 has been identified in vivo and found to alter the interaction of CaMKII with binding partners, but not change its enzymatic activity. Thus, phosphorylation of Thr253 is suggested to modulate functional responses based on its binding partner and subsequently its sub-cellular localization.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot analysis of recombinant FLAG-alpha-CaMKII purified from transfected COS cells. Blots of wild-type CaMKII phosphorylated at Thr253 were treated with lambda phosphatase prior to probing with anti-pT253 or anti-FLAG antibody. The pT253 antibody was incubated with phospho-Thr253 (pT253, 50 nM), non-phospho-Thr253 (T253, 500 nM) or phospho-Thr286 (pT286, 50 nM) peptides prior to probing the western blots. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.

  • Western blots of wild-type CaMKII showing auto-phosphorylation over 4 min at 10 µM ATP, 30°C and probed with antibodies to α-CaMKII, phospho-Thr286-CaMKII (anti-pT286) and phospho-Thr253-CaMKII (anti-pT253). The arrow-heads indicate the electrophoretic mobility of CaMKII phosphorylated at Thr253. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.

    Citations & References

  • Specific References Skelding KA et al (2012) J J Cereb Blood Flow Metab 32 (12), 2181-2192.
    Skelding KA et al (2010) Cell Signal 22 (5), 759-769.
    Gurd JW et al (2008) Brain Res 1218, 158-165.
    Migues PV et al (2006) J Neurochem 98 (1), 289-299.
  • General References Skelding KA et (2011) Cell Cycle 10 (4), 631-63
    Dosemeci A & Jaffe H (2010) Biochem Biophys Res Commun 391 (1), 78-8
    Skelding KA & Rostas JAP (2009) Neurochem Res 34, 1792-180
    Dosemeci, A. et al (1994) J Biol Chem 269 (50), 31330-3133