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Cyclin-dependent kinase inhibitor p27 (p27 KIP 1), Rabbit Polyclonal Antibody
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Catalog Number
R-1121
Discontinued
- Product Name Cyclin-dependent kinase inhibitor p27 (p27 KIP 1), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Cyclin-dependent kinase inhibitor p27 (p27 KIP 1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1; CDKN1B; KIP1;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
- Species Reactivity Human, Mouse (Predicted), Rat
- Immunogen Description A synthetic peptide (NAGSVEQTPKKPGLR) corresponding to a region (180-194) from human p27 KIP 1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Cyclin-dependent kinase inhibitor p27 (p27 KIP 1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human p27 KIP 1 has a predicted length of 198 residues and MW of 22 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Cyclin-dependent kinase inhibitor p27 (p27 KIP 1)
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
- Target Host Species Human
- Species Reactivity Human, Mouse (Predicted), Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (NAGSVEQTPKKPGLR) corresponding to a region (180-194) from human p27 KIP 1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1; CDKN1B; KIP1;
- Uniprot Number P46527
- Uniprot Number/Name P46527 (CDN1B_HUMAN)
- Scientific Background Cyclin-dependent kinase inhibitor p27 (p27 KIP 1) is cell cycle regulatory mitotic inhibitor of cdk activity and an important regulator of cell cycle progression.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.