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Protein phosphatase 2A catalytic subunit alpha (PP2A-alpha), Rabbit Polyclonal Antibody
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Catalog Number
R-1097
- Product Name Protein phosphatase 2A catalytic subunit alpha (PP2A-alpha), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Protein phosphatase 2A catalytic subunit alpha (PP2A-alpha) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; protein phosphatase 2; PP2A-alpha; EC 3.1.3.16; Replication protein C; RP-C; PPP2CA;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
- Species Reactivity Human, Mouse, Rabbit, Rat
- Immunogen Description A synthetic peptide (FTKELDQWIEQLNEC) corresponding to a region (6-20) from human PP2A alpha isoform. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Protein phosphatase 2A catalytic subunit alpha (PP2A-alpha) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human PP2A alpha isoform has a predicted length of 309 residues and MW of 36 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Protein phosphatase 2A catalytic subunit alpha (PP2A-alpha)
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
- Target Host Species Human
- Species Reactivity Human, Mouse, Rabbit, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (FTKELDQWIEQLNEC) corresponding to a region (6-20) from human PP2A alpha isoform. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; protein phosphatase 2; PP2A-alpha; EC 3.1.3.16; Replication protein C; RP-C; PPP2CA;
- Uniprot Number P67775
- Uniprot Number/Name P67775 (PP2AA_HUMAN)
- Scientific Background Serine/threonine-protein phosphatase 2A is one of four major serine and threonine phosphatases and plays an important role in processes such as the regulation of the cell cycle, cell growth and division. It has a common heteromeric core enzyme composed of a catalytic subunit and a constant regulatory subunit. PP2A alpha is the alpha isoform of the catalytic subunit.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.