Eukaryotic translation initiation factor 2C (elF2c), Rabbit Polyclonal Antibody

Product Info

Product Name Eukaryotic translation initiation factor 2C (elF2c), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Eukaryotic translation initiation factor 2C (elF2c) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, IP.
Alternative Names Protein argonaute-1; Protein argonaute-2; Protein argonaute-3; Argonaute1; Argonaute2; Argonaute3; mAgo1; mAgo2; Eukaryotic translation initiation factor 2C; eIF-2C 2; eIF-2C 1; eIF2C 2; Piwi/argonaute family protein meIF2C2 Protein slicer; Eif2c2; Ago1; Ago2;
Application(s) IHC-Frozen, IHC-Paraffin-embedded, IP, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity Specificity has been confirmed by IHC, WB and IP in mouse brain. This antibody is expected to recognise at least elF2c-1, 2 and 3 since they have identical C-termini. Human; mouse; rat; The Ago proteins are highly conserved so cross-reactivity with other species is expected.
Species Reactivity Human, Mouse, Rat
Immunogen Description A synthetic peptide corresponding to a sequence (845-860 aa) from the C-terminus of mouse elF2C conjugated to KLH.
Conjugate Unconjugated
Purity Description Affinity purified
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Eukaryotic translation initiation factor 2C (elF2c) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, IP.
Application(s) IHC-Frozen, IHC-Paraffin-embedded, IP, WB
Application Details Western Blotting (WB), Immunochemistry (IHC) and Immunoprecipitation (IP). The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1,000 to 1:2,000. The elFC2 protein has a predicted length of 860 residues and the MW of the monomer is 94 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Eukaryotic translation initiation factor 2C (elF2c)
Specificity Specificity has been confirmed by IHC, WB and IP in mouse brain. This antibody is expected to recognise at least elF2c-1, 2 and 3 since they have identical C-termini. Human; mouse; rat; The Ago proteins are highly conserved so cross-reactivity with other species is expected.
Target Host Species Mouse
Species Reactivity Human, Mouse, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide corresponding to a sequence (845-860 aa) from the C-terminus of mouse elF2C conjugated to KLH.
Sequence C-KAVQVHQDTLRTMYFA
Purity Description Affinity purified
Format Lyophilized from PBS
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Protein argonaute-1; Protein argonaute-2; Protein argonaute-3; Argonaute1; Argonaute2; Argonaute3; mAgo1; mAgo2; Eukaryotic translation initiation factor 2C; eIF-2C 2; eIF-2C 1; eIF2C 2; Piwi/argonaute family protein meIF2C2 Protein slicer; Eif2c2; Ago1; Ago2;
Uniprot Number Q8CJG0
Uniprot Number/Name Q8CJG0 (AGO2_MOUSE)
Scientific Background The elF2c (Argonaute, Ago) protein is a core protein of the RNA-induced silencing complex (RISC).
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Immunohistochemical staining with Rabbit polyclonal antibody to mouse elF2c (R-1556-100) in(B) mouse hippocampal area. Fixed (4% paraformaldehyde) mouse brain free floating sections were incubated with R-1556-100 at 1 µg/mL overnight followed by incubation with fluoroscein donkey anti-rabbit IgG conjugate at a dilution of 1:100. Scale bar = 20µm.
Immunohistochemical staining with Rabbit polyclonal antibody to mouse elF2c (R-1556-100) in (D) mouse cerebellar cortex. Fixed (4% paraformaldehyde) mouse brain free floating sections were incubated with R-1556-100 at 1 µg/mL overnight followed by incubation with fluoroscein donkey anti-rabbit IgG conjugate at a dilution of 1:100. Scale bar = 20µm.

Western Blot in mouse brain extracts using Rabbit polyclonal to mouse elF2c protein (R-1556-100). Figure A: Total mouse brain extract. Detection at 0.03 µg/mL. Protein loaded 20 µg. Figure B: Mouse cytosolic brain extract. Detection at 0.03 µg/mL. Protein loaded 20 µg, 10 µg and 5 µg.

Citations & References

Specific References Huang KL, Chadee AB, Chen CY, Zhang Y, Shyu AB. (2013)"Phosphorylation at intrinsically disordered regions of PAM2 motif-containing proteins modulates their interactions with PABPC1 and influences mRNA fate." RNA. 2013 Jan 22. [Epub ahead of print].
Lugli G. et al (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem. Jul;106(2):650-61.
Lugli G. et al (2005) Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. Aug;94(4):896-905.