Calbindin, Chicken Polyclonal Antibody

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Catalog Number
C-1798

    Product Info

  • Product Name Calbindin, Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Calbindin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Alternative Names Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type; CALB; CAB27;
  • Application(s) IHC-Frozen, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Human, reacts with human, cow, rat, mouse. Antibody is specific for calbindin and does not recognize closely related proteins parvalbumin and calretinin as determined by Western Blotting.
  • Species Reactivity Bovine, Human, Mouse, Rat
  • Immunogen Description Full-length recombinant human protein
  • Conjugate Unconjugated
  • Purity Description IgY fraction
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Calbindin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Application(s) IHC-Frozen, WB
  • Application Details Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Calbindin
  • Specificity Human, reacts with human, cow, rat, mouse. Antibody is specific for calbindin and does not recognize closely related proteins parvalbumin and calretinin as determined by Western Blotting.
  • Target Host Species Human
  • Species Reactivity Bovine, Human, Mouse, Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Full-length recombinant human protein
  • Purity Description IgY fraction
  • Format Lyophilized IgY preparation, with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type; CALB; CAB27;
  • Uniprot Number P05937
  • Uniprot Number/Name P05937 (CALB1_HUMAN)
  • Scientific Background Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Ref: uniprot.org
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left and Middle: Detection of calbindin-immunoreactivity (red) in adult rat brain cortex (Left) and striatum (Middle) by Immunohistochemistry. The calbindin antibody (1:1,000) labels a subset of sparsely-distributed interneurons (calbindin-positive interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Middle). Since neurons also express Fox3/NeuN (M-377-100, green), calbindin-positive cells appear yellow. Insets are high magnification images of boxed area of each image. Blue: DAPI nuclear stain. IHC method: 45 um sections, fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calbindin expression in rat brain homogenate (Lane 1). The antibody (1:5,000) detects one single band at ~30 kDa. Specificity for calbindin is shown by probing related His-tagged calcium-binding proteins (Lane 2: Parvalbumin; Lane 3: Calretinin; Lane 4: Calbindin; 0.5 ug protein per lane). One additional band at ~60 kDa (Lane 4) most likely represents calbindin protein dimer.

  • Left: Analysis of calbindin expression (green, 1:2,000) in rat cerebellum by Immunohistochemistry. Sections were co-stained with rabbit anti-MeCP2 (R-1810-100, red, 1:5,000). IHC Method: Transcardial perfusion with 4% PFA, post fixation for 24 hours. Sections were cut to 45 um and stained (free-floating). Calbindin, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells. Right: Western blot analysis of rat cerebellum (2), pig hippocampus (3), cow cerebellum (4), parvalbumin (6), calretinin (7), His-tagged calbindin (8). Chicken anti-calbindin (green, 1:5,000) antibody detects calbindin at ~25 kDa in tissue homogenates (2-4). Recombinant human calbindin protein (8) appears at slightly higher molecular weight due to presence of His-tag. The chicken antibody does not cross-react with closely related protein parvalbumin and calretinin.

    Citations & References