Application DetailsWestern blotting (1:5,000-1:20,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunogen DescriptionFull-length recombinant human protein
Purity DescriptionProtein G purified
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundMay participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
Left: Mixed neuron-glial cell cultures stained with anti-CNP (red) and anti-GFAP (green, R-1374-50) by Immunocytochemistry. The antibody to CNP stains strongly in oligodendrocytes, whereas GFAP labels only the intermediate filaments in astrocytes. Blue is DNA staining. Right: Western Blotting of rat brain tissue homogenates probed with anti-CNP antibody at 1:5,000 (Lane 1) and 1:20,000 (Lane 2). The antibody binds strongly and specifically to a band at ~48 kDa corresponding to the expected molecular weight of CNP.
Left: Rat cerebellum stained with mouse anti-CNP (green, 1:500) and chicken anti-NF-M (C-1393-50, red, 1:1,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The CNP antibody specifically stains oligodendrocytes, cells that create the myelin sheath around axons. The NF-M antibody labels axons of neuronal cells. Right: Western blot analysis of rat brain (2), rat spinal cord (3), mouse brain (4), mouse spinal cord (5). Antibody dilution: 1:2,000. Double bands at 46 and 48 kDa correspond to the major isotypes of CNP.
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