Parvalbumin, Mouse Monoclonal Antibody

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Catalog Number
M-1813

    Product Info

  • Product Name Parvalbumin, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Parvalbumin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Alternative Names Parvalbumin alpha
  • Application(s) IHC-Frozen, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Human,reacts with Human, Rat, Mouse. Antibody is specific for parvalbumin and does not recognize closely related proteins calretinin and calbindin as determined by Western Blotting.
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description Full-length recombinant human protein
  • Conjugate Unconjugated
  • Purity Description Protein G purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Parvalbumin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Application(s) IHC-Frozen, WB
  • Application Details Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Note that this antibody does not recognize parvalbumin in rat or mouse brain homogenates on western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Parvalbumin
  • Specificity Human,reacts with Human, Rat, Mouse. Antibody is specific for parvalbumin and does not recognize closely related proteins calretinin and calbindin as determined by Western Blotting.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG1
  • Clone Name 3C9
  • Conjugate Unconjugated
  • Immunogen Description Full-length recombinant human protein
  • Purity Description Protein G purified
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Parvalbumin alpha
  • Uniprot Number P20472
  • Uniprot Number/Name P20472 (PRVA_HUMAN)
  • Scientific Background In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Ref: uniprot.org
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: Staining of parvalbumin in adult rat cerebellum floating section by Immunohistochemistry. Mouse antibody to parvalbumin (1:1,000, green) stains dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue: Nuclear DNA stain. Right: Western blot analysis of parvalbumin expression in rat skeletal muscle homogenate (Lane 1). The antibody (1:1,000) detects parvalbumin at ~12 kDa in homogenate (Lane 1) and His-tagged parvalbumin protein (Lane 2) at slightly higher molecular weight. The antibody does not cross-react with His-tagged proteins calretinin (Lane 3) and calbindin (Lane 4). Note that this antibody does not recognize parvalbumin in rat or mouse brain lysates on western blots.

  • Rat cerebellum section stained with mouse anti-parvalbumin (green, 1:1,000) and chicken anti-calbindin (C-1798-50, red, 1:2,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone, and so appear green.

    Citations & References