Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/mL is recommended for WB. Human Tubulin alpha-1B chain has a predicted length of 451 residues and MW of 50 kDa. A concentration of 1.0-2.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTubulin alpha-1B chain
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
Target Host SpeciesChicken
Species ReactivityChicken, Human, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameTub-1
ConjugateUnconjugated
Immunogen DescriptionMicrotubules from chicken embryo brain
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe tubulin family includes five distinct tubulins; alpha, beta, gamma, delta and epsilon. Microtubules consist principally of alpha- and beta-tubulin.
Specific ReferencesOh H. et al (2013) Uncovering a role for endocannabinoid signaling in autophagy in preimplantation mouse embryos. Mol Hum Reprod. 2013 Feb;19(2):93-101.