Fibrillarin (Nop1p), Mouse Monoclonal Antibody

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Catalog Number
M-1372

    Product Info

  • Product Name Fibrillarin (Nop1p), Mouse Monoclonal Antibody
  • Product Description google
    Mouse anti-rRNA 2'O-methyltransferase fibrillarin (Nop1p) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; Nop1p
  • Application(s) ICC, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity The specificity of this antibody has been confirmed by WB. This clone was selected because it is specific for the ~34 kDa Fibrillarin. It stains a single band on western blotting and shows a clear and strong punctate staining of yeast nuclei. It can therefore be used as a marker for nucleoli in a wide variety of species. Human, Rat, Drosophila, S. pombe, C. elegans and and S. pombe. Predicted to react with other mammalian tissues.
  • Species Reactivity C. elegans, Drosophila, Human, Other Mammals (Predicted), Rat, Yeast
  • Immunogen Description Yeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).
  • Conjugate Unconjugated
  • Purity Description Concentrated cell culture supernatant.
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Mouse anti-rRNA 2'O-methyltransferase fibrillarin (Nop1p) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western Blotting (WB), Immunocytochemistry (ICC). A dilution of 1:2000 (cell lysates) to 1:10000 (nuclear fractions) is recommended for WB of yeast protein samples followed by chemiluminescent detection (ECL). For other non-ECL WB methods, a dilution of 1:1000 to 1:5000 is recommended. A dilution of 1:500 is recommended for ECL WB on mammalian fibrillarin. A dilution of 1:1000 to 1:5000 is recommended for IC on yeast cells and 1:500 for mammalian cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target rRNA 2'O-methyltransferase fibrillarin (Nop1p)
  • Specificity The specificity of this antibody has been confirmed by WB. This clone was selected because it is specific for the ~34 kDa Fibrillarin. It stains a single band on western blotting and shows a clear and strong punctate staining of yeast nuclei. It can therefore be used as a marker for nucleoli in a wide variety of species. Human, Rat, Drosophila, S. pombe, C. elegans and and S. pombe. Predicted to react with other mammalian tissues.
  • Target Host Species Yeast
  • Species Reactivity C. elegans, Drosophila, Human, Other Mammals (Predicted), Rat, Yeast
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 38F3
  • Conjugate Unconjugated
  • Immunogen Description Yeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).
  • Purity Description Concentrated cell culture supernatant.
  • Format Lyophilized hybridoma cell culture media with sodium azide
  • Reconstitution Instructions Spin the vial briefly before opening it. Reconstitute in 250 uL sterile-filtered, room temperature, ultrapure water. Let the vial rehydrate for 5 minutes. Mix gently with a pipette tip to help the redissolution of the material. Centrifuge to remove any insoluble material. The final solution will contain no preservatives. A sterile technique is recommended.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; Nop1p
  • Uniprot Number P15646
  • Uniprot Number/Name P15646 (FBRL_YEAST)
  • Scientific Background Fibrillarin is part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin such as this antibody can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A: Immunofluorescence staining of fibrillarin (Nop1p) expressed in nucleoli with M-1372-250 (green). Cells were double-immunolabelled with an antibody to neurofilament NF-H (red). Blue: cell nucleus, stained with DAPI.
    B: Western blot of whole yeast protein extract with M-1372-250 (left lane). Right lane: no primary antibody control.

  • Left: Visualization of fibrillarin expression (green) in HeLa cells by Immunocytochemistry . Staining was performed with mouse anti-fibrillarin antibody diluted 1:10 from concentrated tissue culture media, and chicken antibody to vimentin (C-1409-50, 1:1,000, red). Blue: DAPI nuclear stain. The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments. Right: Western blot analysis of cell lysates with mouse antibody to fibrillarin (1:500, green). [1] protein standard, [2] C6 cytosol, [3] C6 nucleus, [4] HEK293 cytosol, [5] HEK293 nucleus, [6] NIH-3T3 cytosol and [7] NIH-3T3 nucleus. The band at 37 kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.