Nuclear pore complex protein Nup107, Mouse Monoclonal Antibody

Human HeLa Cells grown in tissue culture and stained with Chicken antibody to Vimentin C-1409-50 (red) and Mouse monoclonal antibody to Nuclear Pore Complex [39C7] M-1397-250 (green). The Vimentin filaments in the cytoplasm of the cells are clearly visible in red, while the nuclear pore complex is also visible in green. The 39C7 antibody recognizes nuclear pore complexes in all eukaryotes tested to date. Picture taken with a Zeiss 40X objective and documented with a Digital SPOT camera.

Left: Analysis of nuclear pore complex (NPC) expression in HeLa cells by Immunocytochemistry. Cells were stained with pan-specific mouse anti-NPC antibody (red, 1:100), and co-stained with chicken antibody to vimentin (C-1409-50, green, 1:10,000). Blue: DAPI nuclear stain. The NPC antibody reveals strong granular staining of the nuclei corresponding to the localization of NPC, while the vimentin antibody labels intermediate filaments in these cells. Right: Western blot analysis of cell lysates for NPC expression (green, lanes 2-7, 1:100), using cytosol- or nuclear-enriched fractions. [1] protein standard, [2] HEK293 cytosol, [3] HEK293 nuclear, [4] NIH-3T3 cytosol, [5] NIH-3T3 nuclear, [6] HeLa cytosol, and [7] HeLa nuclear fraction lysate. The band at about 68 kDa represents a currently unidentified NPC protein which is detected predominantly in the nuclear enriched fractions of all cell lines.