Alternative NamesUbiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1;
Application(s)ICC, IHC-Frozen, WB
Antibody HostMouse
Antibody TypeMonoclonal
SpecificityThe specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord, brain, SHSY-5Y or HEK293 cell extract. Hu, Rat, Bov
Species ReactivityBovine, Human, Mouse, Pig, Rat
Immunogen DescriptionRecombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Application DetailsWestern Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord, brain, SHSY-5Y or HEK293 cell extract. Hu, Rat, Bov
Target Host SpeciesHuman
Species ReactivityBovine, Human, Mouse, Pig, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG1
Clone NameBH7
ConjugateUnconjugated
Immunogen DescriptionRecombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Purity DescriptionProtein G purified
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesUbiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1;
Scientific BackgroundThis enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
An image showing a section of rat spinal cord stained with Mouse monoclonal antibody to Ubiquitin C Terminal Hydrolase 1 [BH7] M-1407-100 (red) and Rabbit polyclonal antibody to neurofilament H R-1388-50 (green). The large cells are a-motorneurons and Ubiquitin C Terminal Hydrolase 1 (UCHL1) fills the cytoplasm of their perikarya and dendrites. The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large motor neurons.
Left: Analysis of UCHL1 expression in rat hippocampal section by Immunohistochemistry. UCHL1 protein was visualized with mouse antibody to UCHL1 (green, 1:5,000), and section co-stained with rabbit anti-FOX3/NeuN (R-3770-100, red, 1:2,000). Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The UCHL1 antibody stains the cell body and dendrites of hippocampal neurons, while the FOX3 antibody labels nuclei of the neuronal cells. Right: Western blot analysis of UCHL1 expression in tissue lysates (green, 1:10,000). [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. The single band at 24 kDa corresponds to the UCHL1 protein.
Ubiquitin C Terminal Hydrolase 1 staining (red) in hippocampal cultures by Immunocytochemistry. Primary antibody dilution: 1:2,000. Blue: nuclear stain. Image courtesy of QBM Cell Science.
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