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Neurofilament light polypeptide, C-terminus, (NF-L-Ct), Rabbit Polyclonal Antibody

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Catalog Number

R-2113

Product Info

Product Name Neurofilament light polypeptide, C-terminus, (NF-L-Ct), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Neurofilament light polypeptide, C-terminus, (NF-L-Ct), Polyclonal Antibody (Unconjugated), suitable for WB and Immunostaining.
Alternative Names NF-L-Ct; NF-L; NF68; NEFL; Neurofilament light polypeptide; NFL; neurofilament light chain; Neurofilament triplet L protein; NFL; c-terminus
Application(s) IF, ICC, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity Species cross-reactivity includes human, rat, mouse, cow, and pig.
Species Reactivity Bovine, Human, Mouse, Pig, Rat
Immunogen Description The antibody has been made against the C-terminal peptide of rat NF-L protein (amino acids 515-543; peptide GEEEDTKESEEEEKKEESAGEEQAAKKKD) with an N-terminal Cys for coupling to KLH.
Conjugate Unconjugated
Purity Description Affinity Purified
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Neurofilament light polypeptide, C-terminus, (NF-L-Ct), Polyclonal Antibody (Unconjugated), suitable for WB and Immunostaining.
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Application(s) IF, ICC, WB
Application Details Western blot (WB), Immunocytochemistry (ICC) / Immunofluorescence (IF). A dilution of 1:10,000-1:20,0000 is recommended for WB. A dilution of 1:5,000 is recommended for ICC/IF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Neurofilament light polypeptide, C-terminus, (NF-L-Ct)
Specificity Species cross-reactivity includes human, rat, mouse, cow, and pig.
Target Host Species Rat
Species Reactivity Bovine, Human, Mouse, Pig, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description The antibody has been made against the C-terminal peptide of rat NF-L protein (amino acids 515-543; peptide GEEEDTKESEEEEKKEESAGEEQAAKKKD) with an N-terminal Cys for coupling to KLH.
Purity Description Affinity Purified
Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names NF-L-Ct; NF-L; NF68; NEFL; Neurofilament light polypeptide; NFL; neurofilament light chain; Neurofilament triplet L protein; NFL; c-terminus
Uniprot Number P07196
Uniprot Number/Name P07196 (NFL_HUMAN)
Scientific Background Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. (Ref: uniprot.org)
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116126
Regulatory Status For research use only.

Images, Protocols & SDS

Image of a rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with R-2113-50, Neurofilament light polypeptide, C-terminus, (NF-L-Ct), Rabbit pAb, (red, 1:5,000), and co-stained with product M-2106-100, Beta-Synuclein, Clone 6A10, Mouse mAb, (green, 1:5,000), and Hoechst staining of nuclear DNA (blue). Method: Following transcardial perfusion of rat with 4% paraformaldehyde brain was fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with antibodies. Product R-2113-50 labels dendrites and axons of the neuronal cells whilst product M-2106-100 detects protein that is concentrated in the synaptic regions.
Analysis by western blot of NF-L expression in different rat and mouse tissue homogenates with R-2113-50, Neurofilament light polypeptide, C-terminus, (NF-L-Ct), Rabbit pAb, (green, 1:20,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: molecular weight marker; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord.

Citations & References

Specific References Bacioglu M et al. (2016) Neurofilament light chain in blood and CSF as a marker of disease progression in mouse models and in neurodegenerative diseases. Neuron. 27292537 Application: Mouse and Human.