Product DescriptiongoogleChicken anti-Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Polyclonal Antibody (Unconjugated), suitable for WB and Immunostaining.
Alternative NamesNeuronal nuclei antigen, NeuN, NA binding protein fox-1 homolog 3, Fox3, NeuN, A6NFN3, RBFOX3, Fox-1 homolog C
Application(s)IF, ICC, IHC, WB
Antibody HostChicken
Antibody TypePolyclonal
SpecificitySpecies cross-reactivity includes human, rat, and mouse.
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionThe antibody has been made against a recombinant construct, based on the N-terminal (100 amino acids) of human FOX3 expressed in and purified from E Coli.
Application DetailsWestern blot (WB), Immunocytochemistry (ICC) / Immunofluorescence (IF), Immunohistochemistry (IHC). A dilution of 1:500 - 1:1,000 is recommended for WB. A dilution of 1:5,000 – 1:10,000 is recommended for ICC/IF and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificitySpecies cross-reactivity includes human, rat, and mouse.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionThe antibody has been made against a recombinant construct, based on the N-terminal (100 amino acids) of human FOX3 expressed in and purified from E Coli.
FormatLyophilized IgY preparation, with sodium azide.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNeuronal nuclei antigen, NeuN, NA binding protein fox-1 homolog 3, Fox3, NeuN, A6NFN3, RBFOX3, Fox-1 homolog C
Scientific BackgroundFOX3 is one of a family of mammalian homologues of FOX1 (FOX is an acronym of "Feminizing locus on X”). The FOX proteins are about 46 kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. Much interest has focused on FOX3 as a result of the recent finding that this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN/FOX3 functions as a Pre-mRNA alternative splicing regulator. It regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD) and is expressed heavily and specifically in neuronal nuclei and cytoplasm. In the cerebellum, Fox3 does not stain Purkinje neurons or Golgi neurons. The antibody was raised against the N-terminal (100 amino acids) of human FOX3, as expressed in and purified from E. coli. The full length FOX3 is not used as the immunogen since the three mammalian FOX homologues, namely FOX1, FOX2 and FOX3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules, so antibodies specific for each of the three molecules can therefore be generated.
Image of a rat hippocampus section by Immunofluorescence and Immunohistochemistry. The section was stained with C-2127-100, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Chicken pAb, (red, 1:5,000), and co-stained with productR-1820-50, Ionized calcium-binding adapter molecule 1 (IBA1), Rabbit pAb, (1:2,000, green). Product C-2127-100 stains the nuclei and distal perikarya of most neurons, while product R-1820-50 labels microglial cells. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, rat brain was post fixed for 24 hours, cut to 35µm, and free floating sections were stained.
Analysis by western blot of NeuN/FOX3 expression in mouse brain nuclear fraction with C-2127-100, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Chicken pAb, (green, 1:1,000) with two bands at approx 46kDa and 50kDa corresponding to the two isoforms of NeuN/FOX3 protein. Lane 1: protein standard (red); Lane 2: mouse brain nuclear fraction.
Image of formalin-fixed paraffin-embedded rat cerebellum section by Immunohistochemistry. The section was stained with C-2127-100, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Chicken pAb, (brown, 1:10,000) and counter stained with Hematoxylin (blue). Product C-2127-100 stains the nuclei and distal perikaryal of most neurons.
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