Image of a rat brain section by Immunofluorescence and Immunohistochemistry. The section was stained with R-3771-50, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Rabbit pAb, (red, 1:2,000), and co-stained with product C-1382-50, Microtubule associated protein (MAP2), Chicken pAb, (green, 1:5,000). Product R-3771-100 selectively stains nuclei and cytoplasm of neuronal cells, while product C-1382-50 labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons. Method: following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained with the two antibodies.