Parvalbumin, Chicken Polyclonal Antibody

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Catalog Number
C-1814

    Product Info

  • Product Name Parvalbumin, Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Parvalbumin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Alternative Names PVALB; Parvalbumin;
  • Application(s) IHC-Frozen, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Human, reacts with Human, Rat, Mouse. Antibody is specific for parvalbumin and does not recognize closely related proteins calretinin and calbindin as determined by Western Blotting.
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description Full-length recombinant human protein
  • Conjugate Unconjugated
  • Purity Description IgY fraction
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Parvalbumin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Application(s) IHC-Frozen, WB
  • Application Details Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Note that this antibody does not recognize parvalbumin in rat or mouse brain homogenates on western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Parvalbumin
  • Specificity Human, reacts with Human, Rat, Mouse. Antibody is specific for parvalbumin and does not recognize closely related proteins calretinin and calbindin as determined by Western Blotting.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Full-length recombinant human protein
  • Purity Description IgY fraction
  • Format Lyophilized IgY preparation, with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names PVALB; Parvalbumin;
  • Uniprot Number P20472
  • Uniprot Number/Name P20472 (PRVA_HUMAN)
  • Scientific Background In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Ref: uniprot.org
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: Staining of parvalbumin in adult rat cerebellum floating section by Immunohistochemistry. Chicken antibody to parvalbumin (1:2,500, green) stains dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue: Nuclear DNA stain. Right: Western blot analysis of parvalbumin expression in mouse skeletal muscle homogenate (Lane 1). The antibody (1:2,500) detects parvalbumin at ~12 kDa in homogenate (Lane 1) and His-tagged parvalbumin protein (Lane 2) at slightly higher molecular weight. The antibody does not cross-react with His-tagged proteins calretinin (Lane 3) and calbindin (Lane 4). Note that this antibody does not recognize parvalbumin in rat or mouse brain lysates on western blots.

  • Rat cerebellum section stained with chicken anti-parvalbumin (green, 1:2,000) and mouse anti-GFAP (red) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free floating sections were stained. The parvalbumin chicken antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astrocytes.

    Citations & References