Product DescriptiongoogleChicken anti-Tyrosine Hydroxylase (TH), Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, FC
Alternative NamesTH; T-H; tyrosine 3-monooxygenase; monooxygenase; oxidoreductase
Application(s)FC, ICC, IHC-Frozen, WB
Antibody HostChicken
Antibody TypePolyclonal
SpecificityHuman. Species cross-reactivity includes rat and mouse.
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionThe antibody has been made against the full length recombinant human TH (524 amino acid sequence) expressed in and purified from E. Coli
Application DetailsWestern Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Flow Cytometry at 20 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityHuman. Species cross-reactivity includes rat and mouse.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionThe antibody has been made against the full length recombinant human TH (524 amino acid sequence) expressed in and purified from E. Coli
Purity DescriptionAffinity-purified
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesTH; T-H; tyrosine 3-monooxygenase; monooxygenase; oxidoreductase
Scientific BackgroundCatalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity). (Ref: uniprot.org)
Left: Visualization of tyrosine hydroxylase expression in rat brain by Immunofluorescent analysis. Section was stained with chicken anti-TH antibody, C-2126-100, (green, 1:5,000) and Hoechst staining of nuclear DNA (blue). Product C-2126-100 stains the dopaminegic TH-positive neurons and their processes of the substantia nigra. Right: Western blot analysis of tissues and lysates using C-2126-100 (green, 1:50,000) with a band at approx. 58kDa in brain tissue lysates. Lane 1: protein standard (red); Lane 2: rat brain caudate/putmen region; Lane 3: rat brain midbrain; Lane 4: whole mouse midbrain; Lane 5: cow midbrain segment.